Tuesday, May 10, 2011

Evolutionary Essay

We've all talked about the study of life before, but have we take the time to really think about how it all flows together? Maybe your theory is different than mine, maybe they're not so different. Either way we all have our own way of thinking how the evolutionary process started, and how it was found out. While doing some research of my own I came across two causes; Comparative morphology and Macroevolution.

Comparative morphology is the systematic study of similarities and differences in body plans between major groups. Scientists started studying this in the 18th century.  By doing this they got a closer look at bone structures and physical appearances of life forms. A study performed to look at comparative morphology would be to compare the pelvic structure of a manatee, but there was no need for a pelvic structure because they don’t walk.  However, they did find out that sea manatees do in fact have a ghost socket where a pelvic structure would be found.  They found out that there was in fact intermediate fossils found that gave evidence to there being such a thing as a “land manatee.”  The fossils contained many of the same characteristics of sea manatees, but their pelvic structured showed that they were able to walk!

Charles Darwin, a great scientists, embarked on a voyage across the sea to South America in 1831 where he studied the diverse life on all the different islands. When Darwin went back to England, he noticed that he had discovered some fossils of extinct animals. It wasn't until he took a good look at them that he realized that some of the new species he examined resembled the extinct animals.  He looked through his drawings and notes and noticed that not all of the species were alike, but they did show variation in size, color, and many other characteristics.  Darwin was able to formulate a theory that included variation, a huge part of Darwin’s conclusion because variation would enable the species to survive and reproduce in certain environments.  

Darwin proposed that an environment would “select” specific characteristics for a species to possess.  This is known today as natural selection. Since then, variations in traits have been shown to be controlled by genes in DNA.  Microevolution occurs when little changes in allele frequencies are brought about by mutation of genes, genetic drift, migration, and natural selection. When mutation in genes occurs, the result is a change in the DNA structure, which is heritable.  This occurrence is the source for diversity in species and organisms.  Gene flow and genetic drift is also a contribution to the diversity of things. When alleles leave or enter a population, as a result of immigration or emigration, it is called gene flow.  This idea is much like genetic drift, however, genetic drift is brought about by chance only.  This size of a population affects the diversity of genetic drift because of the fact that small populations are more likely to loose alleles completely.

Macroevolution is large-scale patterns, trends, and rates at a broader view. Examples that fall under this are fossil records, comparative morphology, and biochemistry. Scientists use sedimentary rock sequences as geological time scales to define eras and eons.  Comparative morphology uses fossils as well as existing life to form similarities that show evolutionary relationships among groups. Scientists identify differences and similarities between species on a molecular level by doing this.  They can look at how one strand of DNA pairs with another from a different species, and form some kind of an idea of the evolutionary distance between the two tested. 

In my opinion, both of these are good studies to the keys to life and evolution.  You can see time and age first, then different structures of species and compare them to modern day species. We are all the same, just look at our DNA! We must have come from one common ancestor. I think that these studies show us a lot about the world, as well as ourselves!

Friday, May 6, 2011

Gel Trays! :)




A few weeks ago we learned how to color dyes into a gel tray. We used a 40 uls micropipet to put the dye into the trays to o see what the scientest used for the different DNA and how it worked is why we used the different color dye. 


In my group we put two red two blues and one green color dyes in. All of them were solid colors but once we plug it in, the electricity will helped us determine which ones are negative and positive energy. It looked really neat watching it happen! After each dye we had to take off the tip of the yellow top because we wanted to make sure you didn't mix up the colors. 


After waiting for 5 minutes the colors started going in different directions or going the same direction.  The dye that went to the right meant that it was negative DNA and the DNA going to the opposite direction ment that it was positive :) It was a pretty neat lab!

Monday, May 2, 2011

Transformation Lab!








This lab was pretty neat! We went through a step-by-step process to figure out a different transformed version of bacteria. For instance we found out if they were more similar to the original non-transformed E. coli colonies. The genetically transformed bacterial cells were transformed after we did these steps (some pictures shown above):

  1. Label one closed micro test tube +pGLO and another -pGLO
  2. Open tubes and use transfer pipet to transfer 250 ul of CaC12
  3. Place tubes on ice
  4. Pick up colony of bacteria with sterile loop
  5. Spin loop in +pGLO tube until colony is dispersed
  6. Place back in ice
  7. Repeat with -pGLO tube
  8. Examine pGLO plasmid DNA solution with UV lamp
  9. Immerse new sterile loop into the plasmid DNA stock tube and withdraw a loopful
  10. Mix loopful into the cell suspension of +pGLO tube
  11. Close and return to ice
  12. Close -pGLO tube but DO NOT add plasmid DNA
  13. Incubate tubes on ice for 10 minutes!
  14. Put both tubes in water bath at 42 degrees C for 50 seconds
  15. Place back in ice for 2 minutes
  16. Remove and open a tube and use new sterile pipet to add 250 ul of LB nutrient broth
  17. Reclose it and repeat with other tube
  18. Incubate tubes for 10 minutes
  19. Pipet 100 ul of the transformation and control suspension onto the plates
  20. Spread suspensions evenly around surface
  21. Stack plates and tape together
  22. Stack and place upside down in 37 degrees C incubator until next day
When we were all done the results were pretty cool! It came out glowing all purple and what not! :) I was surprised because I didn't really think it would look all that cool, but I guess all those steps paid off! It looked awesome! :)